Research

Research of Jia-Ming Chang’s lab: 3D genome organization, single-cell data analysis, protein function prediction, sequence alignment and phylogenetics.

Genome Organization

A topological screen in Drosophila cells lacking CTCF and Cp190 identifies hundreds of insulator elements that modulate local chromatin interactions without driving global genome folding, suggesting Cp190 functions by promoting insulator protein cobinding rather than loop formation [2023, PMID: 36735780]. — Yuri B. Schwartz

Repressed TADs are 3D structural units of chromosome organization in Drosophila [2018, PMID: 295038]. — Giacomo Cavalli

Publications

Sequence Alignment & Phylogenetics

Sampling alignment columns across alternative aligners enhances the discriminative power of bootstrap support regarding tree topology correctness [2019, PMID:30726875]. — Cedric Notredame

The reliability of Multiple Sequence Alignments (MSA) can be robustly quantified using the Transitive Consistency Score (TCS), which is derived from the T-Coffee pairwise library. — Cedric Notredame

Data & Code

  • BigBigTree Web server
    Tree reconstruction for large orthologous families.

Publications

Single-Cell Data Analysis

scGHSOM adapts the Growing Hierarchical Self-Organizing Map (GHSOM) for single-cell data to enable hierarchical clustering and visualization while identifying significant attributes that characterize cell subpopulations [2025, PMID: 40811172]. — Fang Yu

Publications

Computational Protein Function Prediction

Position-Specific Scoring Matrices (PSSMs) contain richer information than primary sequences and can be efficiently generated by searching the UniRef50 database with reduced sensitivity parameters [PMID:24146760].

Dimensionality reduction techniques, such as PLSA, CA, and PCA, not only enhance model performance but also uncover latent relationships within the data [PMID:18260102, PMID:24146760].

Data & Code

  • PSLDoc Code
    Subcellular localization from gapped dipeptides and probabilistic latent semantic analysis.

Publications

Cancer Genomics

Circulating exosomal miRNAs are candidate liquid-biopsy markers for epithelial ovarian cancer. Working with Ramathibodi Hospital, Bangkok, we analysed paired normal and tumour tissue from 45 patients to select miRNAs that separate ovarian cancer subtypes, and reviewed the diagnostic evidence for circulating exosomal miRNAs in the disease [2021, 10.3390/biomedicines9101433]. — Natini Jinawath, Meng-Shin Shiao

Publications